rabbit polyclonal antibody against dcx  (Cell Signaling Technology Inc)

Journal: ACS chemical neuroscience

Article Title: Protective Effects of Intranasally Administrated Oxytocin-Loaded Nanoparticles on Pentylenetetrazole-Kindling Epilepsy in Terms of Seizure Severity, Memory, Neurogenesis, and Neuronal Damage.

doi: 10.1021/acschemneuro.2c00124

Figure Lengend Snippet: Figure 7. Comparison of neurogenesis and apoptosis in the hippocampus. (a) Neurogenesis (doublecortin (DCX) + cells) was higher in the PTZ + NP-OT group when compared to both Ctrl and PTZ groups (*P < 0.05 and ***P < 0.001). (b) Apoptosis (caspase-3 (C-3)++ cells), on the other hand, was increased in the PTZ group when compared to the Ctrl, Ctrl + OT, and Ctrl + NP-OT groups (*P < 0.05). However, the PTZ + OT group showed an increase in apoptosis when compared to Ctrl + OT and Ctrl + NP-OT groups only (*P < 0.05). The PTZ + NP-OT group showed no significant change in apoptosis levels. (c) Representative microphotographs of both DCX+ and C-3+ cells in hippocampus sections counterstained with Mayer’s hematoxylin. Scale bars represent 20 μm. Statistical analyses were performed using the Kruskal−Wallis H test, followed by Dunn’s comparisons of selected group pairs (Ctrl, n = 6; Ctrl + OT, n = 4; Ctrl + NP-OT, n = 4 and 5 for DCX+ and C-3+, respectively; PTZ, n = 8 and 6 for DCX+ and C-3+, respectively; PTZ + OT, n = 5; PTZ + NP-OT, n = 7 and 6 for DCX+ and C-3+, respectively).

Article Snippet: A rabbit polyclonal antibody against doublecortin (DCX) (Biorbyt, U.K., Cat# orb457335, RRID: AB_2904159) was used to demonstrate neurogenesis on the subgranular zone of DG, and a rabbit polyclonal antibody against caspase-3 (C-3) (Sigma-Aldrich, MO, Cat# ab3623, RRID: AB_91556) was used to evaluate apoptosis (Table 1).

Techniques: Comparison